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Image Search Results


Total infection sum score and DNA methylation at birth (suggestive hits) in the discovery dataset (Generation R cohort).

Journal: Brain, behavior, and immunity

Article Title: Prenatal exposure to common infections and newborn DNA methylation: A prospective, population-based study

doi: 10.1016/j.bbi.2024.07.046

Figure Lengend Snippet: Total infection sum score and DNA methylation at birth (suggestive hits) in the discovery dataset (Generation R cohort).

Article Snippet: cg04478698 , − 0.001 , 3.100E-04 , 6.369E-06 , chr4 , 26,033,992 , S_Shelf , -.

Techniques: Infection, DNA Methylation Assay

circSnd1 targets miR-485-3p to regulate inflammation and lipid transport. A, The expression level of circSnd1 after manipulating the expression of circSnd1 and/or miR-485-3p in ox-LDL-induced macrophages using siRNA, miRNA mimic or inhibitor. B, The expression level of miR-485-3p after the alteration of circSnd1 and/or miR-485-3p in ox-LDL-induced macrophages. C-D, The binding sites of circSnd1 by miR-485-3p and its validation by the luciferase reporter experiment. E, Inflammatory factors were detected by ELISA. F, The protein expression of lipid composition and cholesterol transport indicators (Abca1, Abcg1, Ldlr, Hdlbp, Lp-pla2, and Srebp-1c) and Olr1. *, Compared with normal, # compared with model, & compared with si-circRNA. *, #, &, p < 0.05; **, ##, &&, p < 0.01.

Journal: Heliyon

Article Title: circSnd1 promotes atherosclerosis progression through the miR-485-3p/Olr1 signaling pathway

doi: 10.1016/j.heliyon.2023.e17366

Figure Lengend Snippet: circSnd1 targets miR-485-3p to regulate inflammation and lipid transport. A, The expression level of circSnd1 after manipulating the expression of circSnd1 and/or miR-485-3p in ox-LDL-induced macrophages using siRNA, miRNA mimic or inhibitor. B, The expression level of miR-485-3p after the alteration of circSnd1 and/or miR-485-3p in ox-LDL-induced macrophages. C-D, The binding sites of circSnd1 by miR-485-3p and its validation by the luciferase reporter experiment. E, Inflammatory factors were detected by ELISA. F, The protein expression of lipid composition and cholesterol transport indicators (Abca1, Abcg1, Ldlr, Hdlbp, Lp-pla2, and Srebp-1c) and Olr1. *, Compared with normal, # compared with model, & compared with si-circRNA. *, #, &, p < 0.05; **, ##, &&, p < 0.01.

Article Snippet: Primary antibody dilutions (Olr1 (1:1000, DF6522, Affinity), Hdlbp (1:1000, ab133594, Abcam), Ldlr (1:100), Srebp-1c (1:100), Lp-pla2 (1:100), Abcg1 (1:100), Abca1 (1:100), and Gapdh (1:500)) were dropped onto the glass slides and incubated overnight.

Techniques: Expressing, Binding Assay, Luciferase, Enzyme-linked Immunosorbent Assay

Immunohistochemical staining for lipid composition and cholesterol transport indicators (Srebp-1c (a), Lp-pla2 (b), Olr1 (c), Ldlr (d), Hdlbp (e), Abcg1 (f), and Abca1 (g)) in the atherosclerotic lesions of aorta. Scale bar: 50 μm.

Journal: Heliyon

Article Title: circSnd1 promotes atherosclerosis progression through the miR-485-3p/Olr1 signaling pathway

doi: 10.1016/j.heliyon.2023.e17366

Figure Lengend Snippet: Immunohistochemical staining for lipid composition and cholesterol transport indicators (Srebp-1c (a), Lp-pla2 (b), Olr1 (c), Ldlr (d), Hdlbp (e), Abcg1 (f), and Abca1 (g)) in the atherosclerotic lesions of aorta. Scale bar: 50 μm.

Article Snippet: Primary antibody dilutions (Olr1 (1:1000, DF6522, Affinity), Hdlbp (1:1000, ab133594, Abcam), Ldlr (1:100), Srebp-1c (1:100), Lp-pla2 (1:100), Abcg1 (1:100), Abca1 (1:100), and Gapdh (1:500)) were dropped onto the glass slides and incubated overnight.

Techniques: Immunohistochemical staining, Staining